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EXOone Rotavirus A

Reference: RTVA

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Pathogen: Rotavirus A

Target genome
RNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
faeces, rectal swabs and intestine
Endogenous control
Yes

Description

Rotaviruses are a major cause of diarrhea in humans and animals worldwide. They belongs to the family Reoviridae and are taxonomically classified into 8 types (A-H). Its genome is segmented and consists of eleven segments of linear double-stranded RNA.
Rotavirus type A (RVA) is especially known as main cause of severe dirrhoea in livestock such as calves, piglets and foals. Moreover, rotaviruses are considered as zoonotic agents, which might be transmitted between humans and animals.
Several techniques have been implemented to detect this parameter, however, in recent years qPCR have gained importance as rapid, specific and sensitive detection method.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX RTVA_MIX 100 rxns EN ↓ ES ↓
0
oneMIX RTVA_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ RTVA_BEC 100 rxns EN ↓ ES ↓
0
BASIC RTVA_BAS 100 rxns EN ↓ ES ↓
0
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