EXOone Piroplasma

Reference: PIRO

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Pathogen: Piroplasma

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
individual or pooled specimens (up to five) such as blood/serum, liver, spleen, kidney and ticks
Endogenous control
Yes

Description

Piroplasms are intraerythrocytic protozoan parasites mainly belonging to the genera Babesia and Theileria, transmitted by ticks and affecting a wide range of domestic and wild animals.
Infections may cause acute or chronic disease characterized by fever, anemia, jaundice and reduced performance, with variable severity depending on the species and host. These pathogens have an important clinical and economic impact in livestock and are also relevant in companion animals and wildlife.
Conventional detection methods such as microscopy and serology may lack sensitivity, especially in carrier animals or mixed infections. Real-time PCR enables rapid, sensitive and specific detection of piroplasm DNA directly from clinical samples, supporting analytical detection and surveillance.
This assay detects a broad range of Theileria and Babesia species, including clinically relevant agents such as T. equi, T. annulata, T. parva, T. orientalis and T. ovis, as well as B. bovis, B. bigemina, B. caballi, B. canis, B. gibsoni, B. divergens and related species circulating in livestock, pets and wildlife.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX PIRO_MIX 100 rxns EN ↓ ES ↓
0
oneMIX PIRO_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ PIRO_BEC 100 rxns EN ↓ ES ↓
0
BASIC PIRO_BAS 100 rxns EN ↓ ES ↓
0