EXOone Bovine Parainfluenza 3

Reference: PI3V

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Pathogen: Parainfluenza 3 Virus

Target genome
RNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
lungs and nasopharyngeal secretions
Endogenous control
Yes

Description

Bovine Parainfluenza 3 virus belongs to the family Paramyxoviridae and does not usually produce a severe disease in cattle. Latest reports describe this disease as underestimated and endemic. Nevertheless, with other agents makes up the enzootic pneumonia complex which mostly affects calves and causes relevant financial losses.
PI3V infection is usually subclinical but predisposes to secondary bacterial infection and this might lead to clinical signs. Lung tissue or lavages are considered to be appropriate samples but cell culture isolation of this virus is often difficult due to the fragility of the virus. Timing of sampling is crucial to obtain consistent diagnostic results. qPCR allows determining quickly and specifically this agent even in very low titers, thus this technique might be proposed as a powerful tool capable to help in the diagnosis of this complex.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX PI3V_MIX 100 rxns EN ↓ ES ↓
0
oneMIX PI3V_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ PI3V_BEC 100 rxns EN ↓ ES ↓
0
BASIC PI3V_BAS 100 rxns EN ↓ ES ↓
0