Reference: PI3V
Pathogen: Parainfluenza 3 Virus
Description
Bovine Parainfluenza 3 virus belongs to the family Paramyxoviridae and does not usually produce a severe disease in cattle. Latest reports describe this disease as underestimated and endemic. Nevertheless, with other agents makes up the enzootic pneumonia complex which mostly affects calves and causes relevant financial losses.
PI3V infection is usually subclinical but predisposes to secondary bacterial infection and this might lead to clinical signs. Lung tissue or lavages are considered to be appropriate samples but cell culture isolation of this virus is often difficult due to the fragility of the virus. Timing of sampling is crucial to obtain consistent diagnostic results. qPCR allows determining quickly and specifically this agent even in very low titers, thus this technique might be proposed as a powerful tool capable to help in the diagnosis of this complex.
| Reference | Content | Presentation |
|---|---|---|
| oneMIX tube | Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection | 1 or 2 tubes · 750 µl each |
| Positive Control | Lyophilized specific synthetic DNA | 1 tube |
| Water | Molecular grade water | 1 tube (1 ml) |
* 1 tube for 50 reactions format and 2 tubes for 100 reactions format
| Reference | Content | Presentation |
|---|---|---|
| BASIC tube | Lyophilized primers and probe for specific pathogen detection | 1 tube |
| Endogenous Control (EC) BASIC tube | Lyophilized primers and probe for endogenous control detection | 1 tube |
| Positive Control | Lyophilized specific synthetic DNA | 1 tube |
| Buffer A | Reconstitution buffer / Negative control | 1 tube (1.5 ml) |
| Reference | Content | Presentation |
|---|---|---|
| BASIC tube | Lyophilized primers and probe for specific pathogen detection | 1 tube |
| Positive Control | Lyophilized specific synthetic DNA | 1 tube |
| Buffer A | Reconstitution buffer / Negative control | 1 tube (1.5 ml) |
Choose the format you need and add it to your quotation list. Our team will get back to you with a tailored offer.
| Format | Reference | Reactions | Manual | Qty | |
|---|---|---|---|---|---|
| oneMIX | PI3V_MIX | 100 rxns | EN ↓ ES ↓ |
0
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| oneMIX | PI3V_MIX | 50 rxns | EN ↓ ES ↓ |
0
|
|
| BASIC+ | PI3V_BEC | 100 rxns | EN ↓ ES ↓ |
0
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| BASIC | PI3V_BAS | 100 rxns | EN ↓ ES ↓ |
0
|
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