EXOone Ornithobacterium rhinotracheale

Reference: ORNT

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Pathogen: Ornithobacterium rhinotracheale

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
infraorbital sinus swabs, respiratory swabs, trachea, lungs
Endogenous control
Yes

Description

Ornithobacterium rhinotracheale (ORT) is a Gram-negative bacteria (family Flavobacteriaceae) that causes respiratory disease, growth retardation, decreased egg production and mortality in chickens and turkeys. This agent can causes a highly contagious disease in poultry, although the severity of clinical signs, duration of the disease and mortality has been found to be extremely variable. ORT can be a primary or secondary aetiological agent, and this depends on strain virulence, environmental factors, immune status of the flock, and the presence of other infectious agents.
Microbiological culture and serological tests are described for diagnosis of ORT. However, the use of molecular techniques, such as the real-time PCR assay, has showed increased sensitivity and specificity for the identification of this agent.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX ORNT_MIX 100 rxns EN ↓ ES ↓
0
oneMIX ORNT_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ ORNT_BEC 100 rxns EN ↓ ES ↓
0
BASIC ORNT_BAS 100 rxns EN ↓ ES ↓
0