EXOone Erysipelothrix rhusiopathiae

Reference: ERUS

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Pathogen: Erysipelothrix rhusiopathiae

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
synovial fluids, joint tissue, lymph node, liver, lung, spleen
Endogenous control
Yes

Description

Erysipelothrix rhusopathiae is ubiquitous in the environment and has been reported as both saprophyte and etiological cause of disease in a wide range of animals including fish, birds, mammals and human.
Moreover, E. rhusiopathiae is recognized as the etiological agent of swine erysipelas, a disease of significant economic consequence. Four main disease syndromes are reported in porcine infections: acute septicemia, subcutaneous disease (diamond skin), endocarditis and arthritis. Transmission might be mediated by the intake of contaminated food and water or the direct contact between ill animals. Subclinical infected swine should not be neglected due to their potential shedding of the bacteria through their feces, saliva and nasal mucus.
The identification of this bacterium by classical methods might be difficult due to its potential for multiple cellular morphologies and its variable Gram stain results. Therefore the use of an assay for molecular detection improves the sensitivity and specificity of the analysis.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX ERUS_MIX 100 rxns EN ↓ ES ↓
0
oneMIX ERUS_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ ERUS_BEC 100 rxns EN ↓ ES ↓
0
BASIC ERUS_BAS 100 rxns EN ↓ ES ↓
0