EXOone Eimeria spp.

Reference: EIME

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Pathogen: Eimeria spp.

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
faeces, rectal swabs and intestine
Endogenous control
No

Description

Eimeria spp. are apicomplexan protozoan parasites that infect the intestinal tract of a wide range of vertebrate hosts, including poultry, ruminants, rabbits and other domestic animals. These parasites are the causative agents of coccidiosis, one of the most important enteric diseases in animal production worldwide.
Infection may cause diarrhea, dehydration, poor feed conversion, reduced growth performance and increased mortality, particularly in young animals. Disease severity varies according to the Eimeria species involved, parasite burden, host age and immune status, often resulting in significant economic losses in livestock and poultry production.
Conventional detection methods based on microscopic identification of oocysts may be labor-intensive and may not allow accurate species differentiation. Real-time PCR enables rapid, sensitive and specific detection of Eimeria DNA directly from clinical samples, supporting analytical detection and surveillance.

This assay detects a broad range of Eimeria species affecting poultry, ruminants, rabbits and other animal hosts, including E. tenella, E. acervulina, E. maxima, E. necatrix, E. brunetti, E. mitis, E. praecox, E. meleagrimitis, E. adenoeides, E. meleagridis, E. bovis, E. zuernii, E. alabamensis, E. auburnensis, E. ahsata, E. ovinoidalis, E. crandallis, E. parva, E. arloingi, E. ninakohlyakimovae, E. christenseni, E. caprovina, E. stiedai, E. magna, E. intestinalis, E. irresidua, E. perforans and E. media, covering the principal pathogenic species reported in poultry, cattle, sheep, goats and rabbits.

What each tube contains

oneMIX
Reference Content Presentation
ACTB — oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen detection 1 or 2 tubes · 750 µl each
IPCT — Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX EIME_MIX 100 rxns EN ↓ ES ↓
0
oneMIX EIME_MIX 50 rxns EN ↓ ES ↓
0
BASIC EIME_BAS 100 rxns EN ↓ ES ↓
0