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EXOone C. perfringens alpha toxin

Reference: CPTA

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Pathogen: C. perfringens Alpha Toxin

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
faeces, rectal swabs and intestine
Endogenous control
Yes

Description

Clostridium perfringens is associated with a wide variety of diseases in domestic animals and humans. Toxin typing of C. perfringens is important since particular toxin types are associated with specific enteric diseases. Depending on the production of 4 major toxins (alfa, beta, epsilon and iota) they are divided into 5 toxigenic types A to E. Adittionally, other toxins: beta-2- and enterotoxin may contribute to the disease. Bioassays and serological test are used to classify C. perfringens; but, these tests are laborious and expensive. Nowadays, typing is achieved by detecting the encoding gene(s) by qPCR. However it should be considered that presence does not necessarily imply that the encoded protein is actually produced.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX CPTA_MIX 100 rxns EN ↓ ES ↓
0
oneMIX CPTA_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ CPTA_BEC 100 rxns EN ↓ ES ↓
0
BASIC CPTA_BAS 100 rxns EN ↓ ES ↓
0
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