EXOone Clostridium chauvoei

Reference: CHAV

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Pathogen: Clostridium chauvoei

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
organs and blood
Endogenous control
Yes

Description

Clostridium chauvoei is an anaerobic Gram-positive bacterium widely present in soil and capable of surviving for long periods in the environment as spores.
It is the etiological agent of blackleg, a highly fatal disease in cattle and small ruminants characterized by sudden onset, muscle necrosis, gas formation and toxemia, leading to rapid death. The disease causes significant economic losses due to sudden mortality in affected herds.
Conventional detection based on culture may be limited by strict anaerobic requirements and post-mortem sample quality. Real-time PCR enables rapid, sensitive and specific detection of C. chauvoei DNA directly from clinical samples, supporting analytical detection and monitoring.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX CHAV_MIX 100 rxns EN ↓ ES ↓
0
oneMIX CHAV_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ CHAV_BEC 100 rxns EN ↓ ES ↓
0
BASIC CHAV_BAS 100 rxns EN ↓ ES ↓
0