EXOone Clostridium botulinum

Description

Clostridium botulinum is an anaerobic Gram-positive, spore-forming bacterium widely distributed in the environment. Pathogenic strains produce botulinum neurotoxins, which are among the most potent biological toxins known and are responsible for the development of botulism in animals.
Botulism is a severe intoxication characterized by progressive flaccid paralysis, weakness, recumbency and, in advanced cases, respiratory failure and death. The disease affects a wide range of species, including cattle, poultry, horses and companion animals, and can result in significant economic losses due to mortality and production impairment.
Conventional detection methods based on toxin bioassays or bacterial culture may be labor-intensive and time-consuming. Real-time PCR enables rapid, sensitive and specific detection of toxigenic C. botulinum strains through the identification of botulinum neurotoxin-associated genes, supporting analytical detection and surveillance.
This assay is designed for the detection of toxigenic C. botulinum and targets a highly conserved toxin complex gene present in all C. botulinum toxin types and subtypes, providing broad coverage of toxigenic strains associated with animal disease.