EXOone Brachyspira pilosicoli

Reference: BPIL

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Pathogen: Brachyspira pilosicoli

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
faeces, rectal swabs and intestine
Endogenous control
Yes

Description

Brachyspira pilosicoli is an anaerobic intestinal spirochaete which has been isolated from the large intestine or faeces of several animal species such as pigs, chickens, dogs, water birds and human. This bacterium causes colonic spirochetosis which occurs after feco oral transmission.
This brachyspira is frequently found simultaneously with other spirochetes; some of them with reported pathological role like Brachyspira hyodisenteriae but also others with no clearly clinical significance such us Brachyspira intermedia, Brachyspira murdochi or Brachyspira innocens. In those cases detection through microbiological isolation and later identification through biochemical tests might be hard and inefficient. Therefore, a sensitive and specific molecular tool like qPCR allows a quick and precise detection of this bacteria.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

Choose the format you need and add it to your quotation list. Our team will get back to you with a tailored offer.

Format Reference Reactions Manual Qty
oneMIX BPIL_MIX 100 rxns EN ↓ ES ↓
0
oneMIX BPIL_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ BPIL_BEC 100 rxns EN ↓ ES ↓
0
BASIC BPIL_BAS 100 rxns EN ↓ ES ↓
0