Reference: BPIL
Pathogen: Brachyspira pilosicoli
Description
Brachyspira pilosicoli is an anaerobic intestinal spirochaete which has been isolated from the large intestine or faeces of several animal species such as pigs, chickens, dogs, water birds and human. This bacterium causes colonic spirochetosis which occurs after feco oral transmission.
This brachyspira is frequently found simultaneously with other spirochetes; some of them with reported pathological role like Brachyspira hyodisenteriae but also others with no clearly clinical significance such us Brachyspira intermedia, Brachyspira murdochi or Brachyspira innocens. In those cases detection through microbiological isolation and later identification through biochemical tests might be hard and inefficient. Therefore, a sensitive and specific molecular tool like qPCR allows a quick and precise detection of this bacteria.
| Reference | Content | Presentation |
|---|---|---|
| oneMIX tube | Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection | 1 or 2 tubes · 750 µl each |
| Positive Control | Lyophilized specific synthetic DNA | 1 tube |
| Water | Molecular grade water | 1 tube (1 ml) |
* 1 tube for 50 reactions format and 2 tubes for 100 reactions format
| Reference | Content | Presentation |
|---|---|---|
| BASIC tube | Lyophilized primers and probe for specific pathogen detection | 1 tube |
| Endogenous Control (EC) BASIC tube | Lyophilized primers and probe for endogenous control detection | 1 tube |
| Positive Control | Lyophilized specific synthetic DNA | 1 tube |
| Buffer A | Reconstitution buffer / Negative control | 1 tube (1.5 ml) |
| Reference | Content | Presentation |
|---|---|---|
| BASIC tube | Lyophilized primers and probe for specific pathogen detection | 1 tube |
| Positive Control | Lyophilized specific synthetic DNA | 1 tube |
| Buffer A | Reconstitution buffer / Negative control | 1 tube (1.5 ml) |
Choose the format you need and add it to your quotation list. Our team will get back to you with a tailored offer.
| Format | Reference | Reactions | Manual | Qty | |
|---|---|---|---|---|---|
| oneMIX | BPIL_MIX | 100 rxns | EN ↓ ES ↓ |
0
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| oneMIX | BPIL_MIX | 50 rxns | EN ↓ ES ↓ |
0
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| BASIC+ | BPIL_BEC | 100 rxns | EN ↓ ES ↓ |
0
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| BASIC | BPIL_BAS | 100 rxns | EN ↓ ES ↓ |
0
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