EXOone Besnoitia besnoiti

Reference: BESN

Select the format:

Pathogen: Besnoitia besnoiti

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
blood/serum, conjunctival/vaginal secretions, preputial/uterus flushing, semen, tissues and organs including eye, skin, biopsies and uterus
Endogenous control
Yes

Description

Besnoitia besnoiti are cyst-forming apicomplexan parasites which are the causative agent of bovine besnoitiosis, a severe but often non-fatal disease with a significant economic impact in many countries. The disease in cattle is systemic, manifested by swelling of the lymph nodes, subcutaneous edema, diarrhea, abortion and infertility. Detection of acutely infected cattle is difficult since clinical signs are often not characteristic. Diagnosis usually consists in identification of cysts macroscopically or microscopically by histological techniques. There are also IFA or ELISA tests. However, serological methods have the problem that antibodies appear only in a later stage of the infection. Real Time PCRs assays circumvent this issue and allow an specific an early detection procedure.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

Choose the format you need and add it to your quotation list. Our team will get back to you with a tailored offer.

Format Reference Reactions Manual Qty
oneMIX BESN_MIX 100 rxns EN ↓ ES ↓
0
oneMIX BESN_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ BESN_BEC 100 rxns EN ↓ ES ↓
0
BASIC BESN_BAS 100 rxns EN ↓ ES ↓
0