EXOone Actinobacillus suis

Reference: ASUI

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Pathogen: Actinobacillus suis

Target genome
DNA
Technology
Real-Time PCR, Hydrolysis probe
Samples
lungs, respiratory secretions and oral fluids
Endogenous control
Yes

Description

Actinobacillus suis is a beta-haemolytic, Gram-negative bacterium, facultative anaerobe and a common commensal of the tonsils of the soft palate of swine. However, under unknown conditions, it can invade the bloodstream of all ages, resulting in sepcticaemia and sequelae such as meningitis, arthritis and pneumonia.
Diagnosis can be difficult as its clinical signs are similar to other pathogens (Streptococcus suis and Haemophilus parasuis). Besides, isolation of A. suis from clinical specimens may not always be possible for many reasons, such as treatment with antibacterial agents or improper sample handling. Because of that, detection of this pathogen with qPCR tecnique allows a rapid, easy, specific and sensitive diagnosis and for the control of A. suis outbreaks.

What each tube contains

oneMIX
Reference Content Presentation
oneMIX tube Buffer, enzyme, dNTPs and primers/probes for pathogen and EC detection 1 or 2 tubes · 750 µl each
Positive Control Lyophilized specific synthetic DNA 1 tube
Water Molecular grade water 1 tube (1 ml)

* 1 tube for 50 reactions format and 2 tubes for 100 reactions format

BASIC+
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Endogenous Control (EC) BASIC tube Lyophilized primers and probe for endogenous control detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)
BASIC
Reference Content Presentation
BASIC tube Lyophilized primers and probe for specific pathogen detection 1 tube
Positive Control Lyophilized specific synthetic DNA 1 tube
Buffer A Reconstitution buffer / Negative control 1 tube (1.5 ml)

Available formats

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Format Reference Reactions Manual Qty
oneMIX ASUI_MIX 100 rxns EN ↓ ES ↓
0
oneMIX ASUI_MIX 50 rxns EN ↓ ES ↓
0
BASIC+ ASUI_BEC 100 rxns EN ↓ ES ↓
0
BASIC ASUI_BAS 100 rxns EN ↓ ES ↓
0